By Alton Meister
Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, providing researchers entry to authoritative reports of the newest discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, offering an unequalled view of the old improvement of enzymology. The sequence deals researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in complicated organic strategy, and their software in either the laboratory and undefined. each one quantity within the sequence good points contributions by way of prime pioneers and investigators within the box from worldwide. All articles are rigorously edited to make sure thoroughness, caliber, and clarity.
With its wide selection of themes and lengthy historic pedigree, Advances in Enzymology and similar parts of Molecular Biology can be utilized not just via scholars and researchers in molecular biology, biochemistry, and enzymology, but in addition by way of any scientist drawn to the invention of an enzyme, its homes, and its applications.
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Additional info for Advances in Enzymology and Related Areas of Molecular Biology, Volume 47
69. , Debrunner, P. , Marshall, V. J. , and Gunsalus, I . , Biochrmist~,12, 258 (1973). 70. Sligar, S. , Biochrmistry, I S , 5399 (1976). 71. , Eur. J. Bzorhrm. (1977). 72. , and Gunsalus, I. , Eur. J . Biochrni. (1977). 73. , Adu. , I, 157. 74. , Beetlestone, J.. and Griffith, J. ,Rru. Mod. , 36, 441 (1964). 75. Beattie, J . , and West, R. J. Amer. Chem. , 96, 1933 (1974). 76. ,J. A n r r . Chem. , 94, 8607 (1972). 77. Lipscomb, J. D. thesis, University of Illinois, Urbana, Illinois, 1974. 78.
The role of protons in the spin, camphor binding, and redox equilibria can be quantiated by measuring the pH dependence of the various reactions. Figures 15 and 16 show the dependence of the spin equilibrium of mo and mos on proton concentration. The linear relationship favoring the high spin of the enzyme at large Hf con- 28 1. C. GUNSALUS AND S. G. SLIGAR v I Y 40 - my; 7 mys 7 tH+ I I H. m; I Fig. 16. Proton dependence o f the mDSspin equilibria. The in"' spin state is modulated by H + in analogy with that for m" but with a markedly different pK for protonation in the high-spin form.
40. Sligar, S . , and Gunsalus, I. , Proc. Natl. Acad. Sci. U S . , 73, 1078 (1976). 41. Wilson, G. , Tsibris, J . C. , and Gunsalus, I . ,J. B i d . , 248, 6059 (1973). 42. Tsibris, J . C. , and Woody, R. , Coord. Chem. , 5, 417 (1970). 43. Tsibris, J. C. , Tsai, R. , Gunsalus, I. , Orme-Johnson, W. , Hansen, R. , Proc. Natl. Acud. Sci. , 59, 959 (1968). 44. Tsibris, J. C. , Namtvedt, M. , and Gunsalus, 1. C . , Bzorhm. Biophys. Rer. , 30, 323 (1968). 45. Gibson, J. , Hall, D. , Thornley, J . H.